Penter Lab Cancer and immune cell co-evolution at Charité - Universitätsmedizin Berlin

Research

Our research focuses the dissection of mechanisms that underlie response and resistance to immunotherapies and on the longitudinal tracking of tumor cell evolution using single-cell lineage-tracing approaches based on interrogation of natural barcodes like somatic nuclear or mitochondrial DNA mutations.

Highlighted

Integrative genotyping of cancer and immune phenotypes by long-read sequencing
Integrative genotyping of cancer and immune phenotypes by long-read sequencing
Livius Penter, Mehdi Borji, Adi Nagler, Haoxiang Lyu, Wesley S. Lu, ..., Jerome Ritz, Robert J. Soiffer, Jacqueline S. Garcia, Kenneth J. Livak, Catherine J. Wu
Nature Communications   ·   02 Jan 2024   ·   doi:10.1038/s41467-023-44137-7
Targeted genotyping of scRNA-seq cDNA libraries using long-read sequencing with Oxford Nanopore
Mechanisms of response and resistance to combined decitabine and ipilimumab for advanced myeloid disease
Mechanisms of response and resistance to combined decitabine and ipilimumab for advanced myeloid disease
Livius Penter, Yang Liu, Jacquelyn O. Wolff, Lin Yang, Len Taing, ..., Jennifer Altreuter, Franziska Michor, Robert J. Soiffer, Jacqueline S. Garcia, Catherine J. Wu
Blood   ·   13 Apr 2023   ·   doi:10.1182/blood.2022018246
Single cell RNA sequencing analysis dissecting responses to ipilimumab treatment of relapsed AML/MDS with and without stem cell transplantation.
Longitudinal Single-Cell Dynamics of Chromatin Accessibility and Mitochondrial Mutations in Chronic Lymphocytic Leukemia Mirror Disease History
Longitudinal Single-Cell Dynamics of Chromatin Accessibility and Mitochondrial Mutations in Chronic Lymphocytic Leukemia Mirror Disease History
Livius Penter, Satyen H. Gohil, Caleb Lareau, Leif S. Ludwig, Erin M. Parry, ..., Matthew S. Davids, Donna S. Neuberg, Kenneth J. Livak, Vijay G. Sankaran, Catherine J. Wu
Cancer Discovery   ·   01 Dec 2021   ·   doi:10.1158/2159-8290.CD-21-0276
First demonstration that longitudinal changes of mitochondrial DNA mutations reflect therapeutic bottlenecks.

All

2024

Single-cell genomics-based immune and disease monitoring in blood malignancies
Single-cell genomics-based immune and disease monitoring in blood malignancies
Anja C. Rathgeber, Leif S. Ludwig, Livius Penter
Clinical Hematology International   ·   14 Jun 2024   ·   doi:10.46989/001c.117961
Single-cell genomics-based immune and disease monitoring in blood malignancies
Integrative genotyping of cancer and immune phenotypes by long-read sequencing
Integrative genotyping of cancer and immune phenotypes by long-read sequencing
Livius Penter, Mehdi Borji, Adi Nagler, Haoxiang Lyu, Wesley S. Lu, ..., Jerome Ritz, Robert J. Soiffer, Jacqueline S. Garcia, Kenneth J. Livak, Catherine J. Wu
Nature Communications   ·   02 Jan 2024   ·   doi:10.1038/s41467-023-44137-7
Targeted genotyping of scRNA-seq cDNA libraries using long-read sequencing with Oxford Nanopore

2023

Preexisting tumor-resident T cells with cytotoxic potential associate with response to neoadjuvant anti–PD-1 in head and neck cancer
Preexisting tumor-resident T cells with cytotoxic potential associate with response to neoadjuvant anti–PD-1 in head and neck cancer
Giacomo Oliveira, Ann Marie Egloff, Alexander B. Afeyan, Jacquelyn O. Wolff, Zexiang Zeng, ..., Dorina Kallogjeri, Jay F. Piccirillo, Douglas R. Adkins, Catherine J. Wu, Ravindra Uppaluri
Science Immunology   ·   29 Sep 2023   ·   doi:10.1126/sciimmunol.adf4968
First demonstration that preexisting tumor-resident T cells associate with response to neoadjuvant anti-PD-1 in head and neck cancer
Mechanisms of response and resistance to combined decitabine and ipilimumab for advanced myeloid disease
Mechanisms of response and resistance to combined decitabine and ipilimumab for advanced myeloid disease
Livius Penter, Yang Liu, Jacquelyn O. Wolff, Lin Yang, Len Taing, ..., Jennifer Altreuter, Franziska Michor, Robert J. Soiffer, Jacqueline S. Garcia, Catherine J. Wu
Blood   ·   13 Apr 2023   ·   doi:10.1182/blood.2022018246
Single cell RNA sequencing analysis dissecting responses to ipilimumab treatment of relapsed AML/MDS with and without stem cell transplantation.
Ipilimumab plus decitabine for patients with MDS or AML in posttransplant or transplant-naïve settings
Ipilimumab plus decitabine for patients with MDS or AML in posttransplant or transplant-naïve settings
Jacqueline S. Garcia, Yael Flamand, Livius Penter, Michael Keng, Benjamin K. Tomlinson, ..., Catherine J. Wu, Daniel J. DeAngelo, Donna Neuberg, Richard M. Stone, Robert J. Soiffer
Blood   ·   13 Apr 2023   ·   doi:10.1182/blood.2022017686
Clinical study of CTLA-4 blockade and decitabine in relapsed AML/MDS with or without stem cell transplantation.

2022

<i>In Vivo</i>Modeling of CLL Transformation to Richter Syndrome Reveals Convergent Evolutionary Paths and Therapeutic Vulnerabilities
In VivoModeling of CLL Transformation to Richter Syndrome Reveals Convergent Evolutionary Paths and Therapeutic Vulnerabilities
Elisa ten Hacken, Tomasz Sewastianik, Shanye Yin, Gabriela Brunsting Hoffmann, Michaela Gruber, ..., Kenneth J. Livak, Ivana Bozic, Donna S. Neuberg, Ruben D. Carrasco, Catherine J. Wu
Blood Cancer Discovery   ·   06 Dec 2022   ·   doi:10.1158/2643-3230.BCD-22-0082
Mitochondrial DNA Mutations as Natural Barcodes for Lineage Tracing of Murine Tumor Models
Mitochondrial DNA Mutations as Natural Barcodes for Lineage Tracing of Murine Tumor Models
Livius Penter, Elisa ten Hacken, Jackson Southard, Caleb A. Lareau, Leif S. Ludwig, Shuqiang Li, Donna S. Neuberg, Kenneth J. Livak, Catherine J. Wu
Cancer Research   ·   05 Dec 2022   ·   doi:10.1158/0008-5472.CAN-22-0275
First demonstration of mtDNA mutation-based lineage-tracing in murine cancer models.
Immune Phenotypes and Target Antigens of Clonally Expanded Bone Marrow T Cells in Treatment-Naïve Multiple Myeloma
Immune Phenotypes and Target Antigens of Clonally Expanded Bone Marrow T Cells in Treatment-Naïve Multiple Myeloma
Carlotta Welters, María Fernanda Lammoglia Cobo, Christian Alexander Stein, Meng-Tung Hsu, Amin Ben Hamza, ..., Holger Hackstein, Hans-Peter Rahn, Klaus Dornmair, Thomas Blankenstein, Leo Hansmann
Cancer Immunology Research   ·   19 Sep 2022   ·   doi:10.1158/2326-6066.CIR-22-0434
AML relapse after a TIGIT race
AML relapse after a TIGIT race
Livius Penter, Catherine J. Wu
Blood   ·   15 Sep 2022   ·   doi:10.1182/blood.2022017614
Comment on Gournay et al. describing post-transplant immune reconstitution dynamics.
Rapid single-cell identification of Epstein–Barr virus-specific T-cell receptors for cellular therapy
Rapid single-cell identification of Epstein–Barr virus-specific T-cell receptors for cellular therapy
María Fernanda Lammoglia Cobo, Carlotta Welters, Leonie Rosenberger, Matthias Leisegang, Kerstin Dietze, ..., Klaus Dornmair, Thomas Blankenstein, Thomas Kammertöns, Armin Gerbitz, Leo Hansmann
Cytotherapy   ·   01 Aug 2022   ·   doi:10.1016/j.jcyt.2022.03.005
Improved T-cell Immunity Following Neoadjuvant Chemotherapy in Ovarian Cancer
Improved T-cell Immunity Following Neoadjuvant Chemotherapy in Ovarian Cancer
Min Liu, Nabihah Tayob, Livius Penter, MacLean Sellars, Anna Tarren, ..., Guanglan Zhang, Ursula A. Matulonis, Catherine J. Wu, Derin B. Keskin, Panagiotis A. Konstantinopoulos
Clinical Cancer Research   ·   20 Apr 2022   ·   doi:10.1158/1078-0432.CCR-21-2834
Natural Barcodes for Longitudinal Single Cell Tracking of Leukemic and Immune Cell Dynamics
Natural Barcodes for Longitudinal Single Cell Tracking of Leukemic and Immune Cell Dynamics
Livius Penter, Satyen H. Gohil, Catherine J. Wu
Frontiers in Immunology   ·   03 Jan 2022   ·   doi:10.3389/fimmu.2021.788891

2021

Longitudinal Single-Cell Dynamics of Chromatin Accessibility and Mitochondrial Mutations in Chronic Lymphocytic Leukemia Mirror Disease History
Longitudinal Single-Cell Dynamics of Chromatin Accessibility and Mitochondrial Mutations in Chronic Lymphocytic Leukemia Mirror Disease History
Livius Penter, Satyen H. Gohil, Caleb Lareau, Leif S. Ludwig, Erin M. Parry, ..., Matthew S. Davids, Donna S. Neuberg, Kenneth J. Livak, Vijay G. Sankaran, Catherine J. Wu
Cancer Discovery   ·   01 Dec 2021   ·   doi:10.1158/2159-8290.CD-21-0276
First demonstration that longitudinal changes of mitochondrial DNA mutations reflect therapeutic bottlenecks.
Coevolving JAK2V617F+relapsed AML and donor T cells with PD-1 blockade after stem cell transplantation: an index case
Coevolving JAK2V617F+relapsed AML and donor T cells with PD-1 blockade after stem cell transplantation: an index case
Livius Penter, Satyen H. Gohil, Teddy Huang, Emily M. Thrash, Dominik Schmidt, ..., F. Stephen Hodi, Kenneth J. Livak, Robert Zeiser, Pavan Bachireddy, Catherine J. Wu
Blood Advances   ·   18 Nov 2021   ·   doi:10.1182/bloodadvances.2021004335
Integrated analysis of exceptional response to PD-1 inhibition in relapsed sAML after allogeneic stem cell transplantation with scRNA-seq, CITE-seq, scATAC-seq and CyTOF.
Molecular and cellular features of CTLA-4 blockade for relapsed myeloid malignancies after transplantation
Molecular and cellular features of CTLA-4 blockade for relapsed myeloid malignancies after transplantation
Livius Penter, Yi Zhang, Alexandra Savell, Teddy Huang, Nicoletta Cieri, ..., Robert J. Soiffer, X. Shirley Liu, Matthew S. Davids, Pavan Bachireddy, Catherine J. Wu
Blood   ·   16 Mar 2021   ·   doi:10.1182/blood.2021010867
Dissection of exceptional responses to CTLA-4 blockade in relapsed leukemia cutis after allogeneic stem cell transplantation.

2020

Personal tumor antigens in blood malignancies: genomics-directed identification and targeting
Personal tumor antigens in blood malignancies: genomics-directed identification and targeting
Livius Penter, Catherine J. Wu
Journal of Clinical Investigation   ·   27 Jan 2020   ·   doi:10.1172/JCI129209
Review of targets for cellular immunotherapies in blood malignancies.

2019

Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)
Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)
Andrea Cossarizza, Hyun‐Dong Chang, Andreas Radbruch, Andreas Acs, Dieter Adam, ..., Yi Zhao, Susanne Maria Ziegler, Christina Zielinski, Jakob Zimmermann, Arturo Zychlinsky
European Journal of Immunology   ·   01 Oct 2019   ·   doi:10.1002/eji.201970107
Best practices for flow cytometry.
Localization-associated immune phenotypes of clonally expanded tumor-infiltrating T cells and distribution of their target antigens in rectal cancer
Localization-associated immune phenotypes of clonally expanded tumor-infiltrating T cells and distribution of their target antigens in rectal cancer
Livius Penter, Kerstin Dietze, Julia Ritter, Maria Fernanda Lammoglia Cobo, Josefin Garmshausen, ..., Sandra Wienzek-Lischka, Thomas Blankenstein, Michael Hummel, Klaus Dornmair, Leo Hansmann
OncoImmunology   ·   24 Mar 2019   ·   doi:10.1080/2162402X.2019.1586409
Phenotypes and T cell receptor sequences of tumor-infiltrating lymphocytes compared to physiologic tissue-resident T cells.

2018

FACS single cell index sorting is highly reliable and determines immune phenotypes of clonally expanded T cells
FACS single cell index sorting is highly reliable and determines immune phenotypes of clonally expanded T cells
Livius Penter, Kerstin Dietze, Lars Bullinger, Jörg Westermann, Hans‐Peter Rahn, Leo Hansmann
European Journal of Immunology   ·   03 Apr 2018   ·   doi:10.1002/eji.201847507
Demonstration of the high accuracy of FACS index sorting using single T and B cell receptor sequencing.

2017

Clonal Expansion and Interrelatedness of Distinct B-Lineage Compartments in Multiple Myeloma Bone Marrow
Clonal Expansion and Interrelatedness of Distinct B-Lineage Compartments in Multiple Myeloma Bone Marrow
Leo Hansmann, Arnold Han, Livius Penter, Michaela Liedtke, Mark M. Davis
Cancer Immunology Research   ·   31 Aug 2017   ·   doi:10.1158/2326-6066.cir-17-0012
Identification of myeloma clones in phenotypically healthy B cell compartment using B cell receptor sequencing.

2015

Ruxolitinib in corticosteroid-refractory graft-versus-host disease after allogeneic stem cell transplantation: a multicenter survey
Ruxolitinib in corticosteroid-refractory graft-versus-host disease after allogeneic stem cell transplantation: a multicenter survey
R Zeiser, A Burchert, C Lengerke, M Verbeek, K Maas-Bauer, ..., C Peschel, A Neubauer, J Finke, J Duyster, N von Bubnoff
Leukemia   ·   31 Jul 2015   ·   doi:10.1038/leu.2015.212
A rapid screening system evaluates novel inhibitors of DNA methylation and suggests F-box proteins as potential therapeutic targets for high-risk neuroblastoma
A rapid screening system evaluates novel inhibitors of DNA methylation and suggests F-box proteins as potential therapeutic targets for high-risk neuroblastoma
Livius Penter, Bert Maier, Ute Frede, Benjamin Hackner, Thomas Carell, Christian Hagemeier, Matthias Truss
Targeted Oncology   ·   06 Jan 2015   ·   doi:10.1007/s11523-014-0354-5